The GasPak™ EZ Gas Generating Pouch Systems are single-use systems that produce atmospheres suitable to support the primary isolation and cultivation of anaerobic, microaerophilic, or capnophilic bacteria by use of gas generating sachets inside single-use resealable pouches.
SUMMARY AND EXPLANATION In 1965, Brewer and Allgeier introduced a disposable hydrogen generator envelope, which was later modified to include carbon dioxide generation and an internal catalyst. The GasPak EZ Gas Generating Pouch Systems offer bio-performance and reliability without the need for a catalyst or an activation procedure. The GasPak EZ Gas Generating Sachets contain all the ingredients needed to create a specific atmosphere for specimen incubation. The sachet and specimens are placed in the GasPak EZ resealable pouch. The pouch is sealed and incubated.
PRINCIPLES OF THE PROCEDURE The GasPak EZ Gas Generating Sachet consists of a reagent sachet containing inorganic carbonate, activated carbon, ascorbic acid and water. When the sachet is removed from the outer wrapper, the sachet becomes activated by exposure to air. The activated reagent sachet and specimens are placed in the resealable pouch and sealed. The sachet rapidly reduces the oxygen concentration within the pouch. At the same time, inorganic carbonate produces carbon dioxide. For the cultivation of anaerobic bacteria, the GasPak EZ Anaerobe Pouch System with Indicator Sachets produce an anaerobic atmosphere within 2.5 h with greater than or equal to 10% carbon dioxide within 24 h. For the cultivation of microaerophilic bacteria, the GasPak EZ Campy Pouch System Sachets produce an atmosphere with approximately 5 – 15% oxygen. For the cultivation of carbon dioxide requiring organisms, the GasPak EZ CO2 Pouch System Sachets produce an atmosphere with greater than 3% carbon dioxide.
GasPak™ EZ CO2 Pouch System
Pack of 20
BD BBL 260684